Effects of 10 daily doses of ethanol on IL-10 in brain and liver at 1 week after LPS treatment. Male C57BL/6J mice were treated intragastrically with ethanol (5 g/kg, i.g.) for 10 days and were injected intraperitoneally (i.p.) with LPS at the indicated doses 24 hrs after ethanol treatment. Brains and livers were collected at 1 week after LPS injection. The level of IL-10 protein was measured by ELISA. (A) Ten doses of ethanol decreased brain IL-10 and enhanced LPS-induced decrease in IL-10 in a LPS dose-dependent manner at 1 week after LPS treatment. (B) There was a significant increase in liver IL-10 protein in 10 daily doses of ethanol, LPS (0.3 and 3 mg/kg) and ETOH-LPS groups. P < 0.05, ** P < 0.01, compared with the saline controls.# P < 0.05, ## P < 0.01, compared with the corresponding LPS-treated group.