Increased systemic and brain cytokine production and neuroinflammation by endotoxin following ethanol treatment

Table 1 Ten daily doses of ethanol and LPS induced changes in gene expression in brain and liver

Gene	Control	ETOH	LPS	ETOH + LPS
Brain
TNFα	100 ± 19	362 ± 73**	9147 ± 3080*	9229 ± 1464*
MCP-1	100 ± 5	130 ± 7**	618 ± 156**	559 ± 186*
IL-1β	100 ± 9	133 ± 20	4172 ± 1115**	3962 ± 651*
COX-2	100 ± 10	82 ± 8	459 ± 43**	487 ± 72**
gp91^phox	100 ± 11	194 ± 19**	155 ± 11**	239 ± 31**#
p67^phox	100 ± 7	144 ± 11	106 ± 9	138 ± 29*
IL-10	100 ± 29	254 ± 72	5776 ± 1633*	7006 ± 2840
Liver
TNFα	100 ± 10	41 ± 7**	8560 ± 1284**	21238 ± 4810**##
MCP-1	100 ± 7	53 ± 3**	27787 ± 1580**	29249 ± 2338**
IL-1β	100 ± 21	27 ± 3**	6477 ± 691**	7197 ± 1647**
COX-2	100 ± 9	81 ± 24	12335 ± 1128**	6317 ± 707**
gp91^phox	100 ± 7	119 ± 14	187 ± 13**	261 ± 22**#
p67^phox	100 ± 9	108 ± 13	1 04 ± 17	129 ± 31
IL-10	100 ± 7	64 ± 10*	11428 ± 1543**	5625 ± 922**

Ten daily doses of ethanol and LPS induced changes in gene expression in brain and liver. Male C57BL/6J mice were treated intragastrically with ethanol (5 g/kg, i.g.) daily for 10 days and injected intraperitoneally (i.p.) with LPS (3 mg/kg) 24 hrs after ethanol treatment. Brain and liver samples were collected 1 hr post LPS treatment. Gene expression of proinflammatory and anti-inflammatory factors was measured by real-time PCR. The results are the means ± SEM (n = 6 per group). * P < 0.05, ** P < 0.01, compared with the saline controls. ^# P < 0.05, ^## P < 0.01, compared with LPS-treated group.

ISSN: 1742-2094