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Table 1 Ten daily doses of ethanol and LPS induced changes in gene expression in brain and liver

From: Increased systemic and brain cytokine production and neuroinflammation by endotoxin following ethanol treatment

Gene Control ETOH LPS ETOH + LPS
Brain     
TNFα 100 ± 19 362 ± 73** 9147 ± 3080* 9229 ± 1464*
MCP-1 100 ± 5 130 ± 7** 618 ± 156** 559 ± 186*
IL-1β 100 ± 9 133 ± 20 4172 ± 1115** 3962 ± 651*
COX-2 100 ± 10 82 ± 8 459 ± 43** 487 ± 72**
gp91phox 100 ± 11 194 ± 19** 155 ± 11** 239 ± 31**#
p67phox 100 ± 7 144 ± 11 106 ± 9 138 ± 29*
IL-10 100 ± 29 254 ± 72 5776 ± 1633* 7006 ± 2840
Liver     
TNFα 100 ± 10 41 ± 7** 8560 ± 1284** 21238 ± 4810**##
MCP-1 100 ± 7 53 ± 3** 27787 ± 1580** 29249 ± 2338**
IL-1β 100 ± 21 27 ± 3** 6477 ± 691** 7197 ± 1647**
COX-2 100 ± 9 81 ± 24 12335 ± 1128** 6317 ± 707**
gp91phox 100 ± 7 119 ± 14 187 ± 13** 261 ± 22**#
p67phox 100 ± 9 108 ± 13 1 04 ± 17 129 ± 31
IL-10 100 ± 7 64 ± 10* 11428 ± 1543** 5625 ± 922**
  1. Ten daily doses of ethanol and LPS induced changes in gene expression in brain and liver. Male C57BL/6J mice were treated intragastrically with ethanol (5 g/kg, i.g.) daily for 10 days and injected intraperitoneally (i.p.) with LPS (3 mg/kg) 24 hrs after ethanol treatment. Brain and liver samples were collected 1 hr post LPS treatment. Gene expression of proinflammatory and anti-inflammatory factors was measured by real-time PCR. The results are the means ± SEM (n = 6 per group). * P < 0.05, ** P < 0.01, compared with the saline controls. # P < 0.05, ## P < 0.01, compared with LPS-treated group.