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Table 2 A single dose of ethanol and LPS induced changes in gene expression in brain and liver

From: Increased systemic and brain cytokine production and neuroinflammation by endotoxin following ethanol treatment

Gene

Control

ETOH

LPS

ETOH + LPS

Brain

    

TNFα

100 ± 13

413 ± 100*

4595 ± 1360*

19079 ± 6532*

MCP-1

100 ± 5

146 ± 12**

291 ± 65

1294 ± 215*

IL-1β

100 ± 15

91 ± 12

1536 ± 385*

4534 ± 810**#

COX-2

100 ± 6

154 ± 13**

386 ± 41**

474 ± 44**

gp91phox

100 ± 9

127 ± 9

138 ± 19

228 ± 44*#

p67phox

100 ± 10

131 ± 12

116 ± 10

98 ± 18

IL-10

100 ± 13

59 ± 20

1578 ± 825

4864 ± 873*

Liver

    

TNFα

100 ± 7

121 ± 30

13632 ± 2417**

13894 ± 984**

MCP-1

100 ± 9

86 ± 10

13279 ± 2004**

21332 ± 5143**

IL-1β

100 ± 15

20 ± 2**

2215 ± 211**

2232 ± 457**

COX-2

100 ± 27

136 ± 11

9663 ± 1056**

8296 ± 467**

gp91phox

100 ± 12

62 ± 5*

125 ± 12

131 ± 25

p67phox

100 ± 15

61 ± 4*

106 ± 18

81 ± 14

IL-10

100 ± 20

26 ± 4**

3615 ± 394**

4854 ± 979**

  1. Ethanol and LPS induced changes in gene expression in brain and liver. Male C57BL/6J mice were treated intragastrically with ethanol (5 g/kg, i.g.) for one day. Mice were injected intraperitoneally (i.p.) with LPS (3 mg/kg) 24 hrs after ethanol treatment. Brain and liver samples were collected at 1 hr post LPS treatment. Gene expression of proinflammatory and anti-inflammatory factors was measured by real-time PCR. The results are the means ± SEM (n = 6 per group). * P < 0.05, ** P < 0.01, compared with the saline controls. # P < 0.05 compared with LPS-treated group.