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Table 2 A single dose of ethanol and LPS induced changes in gene expression in brain and liver

From: Increased systemic and brain cytokine production and neuroinflammation by endotoxin following ethanol treatment

Gene Control ETOH LPS ETOH + LPS
Brain     
TNFα 100 ± 13 413 ± 100* 4595 ± 1360* 19079 ± 6532*
MCP-1 100 ± 5 146 ± 12** 291 ± 65 1294 ± 215*
IL-1β 100 ± 15 91 ± 12 1536 ± 385* 4534 ± 810**#
COX-2 100 ± 6 154 ± 13** 386 ± 41** 474 ± 44**
gp91phox 100 ± 9 127 ± 9 138 ± 19 228 ± 44*#
p67phox 100 ± 10 131 ± 12 116 ± 10 98 ± 18
IL-10 100 ± 13 59 ± 20 1578 ± 825 4864 ± 873*
Liver     
TNFα 100 ± 7 121 ± 30 13632 ± 2417** 13894 ± 984**
MCP-1 100 ± 9 86 ± 10 13279 ± 2004** 21332 ± 5143**
IL-1β 100 ± 15 20 ± 2** 2215 ± 211** 2232 ± 457**
COX-2 100 ± 27 136 ± 11 9663 ± 1056** 8296 ± 467**
gp91phox 100 ± 12 62 ± 5* 125 ± 12 131 ± 25
p67phox 100 ± 15 61 ± 4* 106 ± 18 81 ± 14
IL-10 100 ± 20 26 ± 4** 3615 ± 394** 4854 ± 979**
  1. Ethanol and LPS induced changes in gene expression in brain and liver. Male C57BL/6J mice were treated intragastrically with ethanol (5 g/kg, i.g.) for one day. Mice were injected intraperitoneally (i.p.) with LPS (3 mg/kg) 24 hrs after ethanol treatment. Brain and liver samples were collected at 1 hr post LPS treatment. Gene expression of proinflammatory and anti-inflammatory factors was measured by real-time PCR. The results are the means ± SEM (n = 6 per group). * P < 0.05, ** P < 0.01, compared with the saline controls. # P < 0.05 compared with LPS-treated group.