Real-time quantitative PCR study. TNF-α (A) and IL-1β (B) transcripts in 12 regions of the rabbit CNS at 24 h after injection of PBS(-) as a calibrator (open bar: 1 ± SD) or 2.5 μg/kg Stx2 (closed bar: mean ± SD). All data were normalized to the internal reference GAPDH amounts and expressed as an n-fold increase relative to normalized calibrator values in each region. Both TNF-α and IL-1β mRNA levels were greatly up-regulated. In particular, TNF-α transcripts were significantly overexpressed in the cerebrum, basal ganglia, hippocampus, thalamus, and cerebral cortex. *: P < 0.01 compared to frontal region.