Double immunostaining of IgG extravasation and the expression of ZO-1. Cholesterol-enriched diet markedly decreased the immunopositive staining of ZO-1 (green) at the sites where IgG extravasation (red) was present. These effects were blocked by chronic ingestion of caffeine. Representative images taken from 2 rabbits in each group with 6 sections from each animal are shown. Bar = 20 μm.