Double immunostaining of IgG extravasation and the expression of GFAP. Cholesterol-enriched diet markedly enhanced the immunopositive staining of GFAP (green) around the sites where IgG extravasation (red) was present. These effects were blocked by caffeine at the dose of 3 mg/day. Representative images taken from 2 rabbits in each group with 6 sections from each animal are shown. Bar = 20 μm.