Figure 5
CD11c positive microglia (green immunofluorescence) are present in TGSED and colocalize with Iba-1 (arrowheads) but do not appear vascular (A). CD11c labeling in TGRUN appeared in cells not labeled by Iba-1 (red) that were linearly arranged, perhaps within or around microvessels. (B, D). Larger vessels had CD11c labeling along the vessel wall, perhaps in the perivascular space (C). Using macrophage markers CD68, we observed microvascular labeling again only in TGRUN (D). Double labeling for CD11c (green) and CD68 (red) revealed that CD11c+ cells were adjacent to CD68+ cells in and around vasculature (arrows) (E, F). Using mannose receptor antibody (red), specific for perivascular macrophages, we again observed vascular labeling only in TGRUN (G-I). High magnification shows the mannose receptor labeled cells are within vessels (H, I)(arrowheads). Green indicates Iba-1 labeling for microglia in and around vessels (G-I).