Figure 7
CDDO-Me enhances phagocytic activity of BV2 microglia induced by TNF or LPS + fibrillar AÎČ 1â42 peptide. BV2 microglia were plated, switched to serum-free media 24 hours later, and stimulated overnight as indicated (LPS, 10 ng/mL; TNF, 10 ng/mL; fAÎČ42, 1 ÎŒM; and CDDO-Me, 10 nM.). Fluorescently-labeled E. coli particles were added to the cultures for 2 hours following stimulation and phagocytosis of particles was measured by fluorescence emission at 520 nm. Values represent mean phagocytic activity relative to baseline (vehicle-stimulated control) ± SEM. Values were analyzed by two-way ANOVA followed by Tukey's post hoc test, * denotes CDDO-Me is significantly different from its DMSO vehicle for a given treatment; # denotes significant difference from the DMSO vehicle in unstimulated cells; + denotes significant difference from CDDO-Me in stimulated cells; all symbols at p < 0.05.