Figure 2

LPS-induced TLR2 surface expression on microglia is reduced by minocycline. Adult mice were injected i.p. with vehicle or minocycline for three consecutive days. On the third day mice were challenged with saline or LPS i.p., microglia and macrophages were collected 24 h later and TLR2 surface expression was determined. A) Representative bivariate density plot of stained cells. Macrophages were CD11b⁺/CD45^high and microglia were CD11b⁺/CD45^low. B&C) Representative histograms of TLR2 expression on microglia following experimental treatments. C) Mean fluorescence intensity (M.F.I.) of TLR2 expression on microglia (CD11b⁺/CD45^low) following experimental treatments. Bars represent the mean ± SEM (n = 6). Means with different letters (a, b, or c) are significantly different (P < 0.05) from each other.