Figure 4

Effects of COX-2 deficiency on LPS-induced expression of enzymes involved in the arachidonic acid cascade. Quantitative real time-PCR analysis of mPGES-1 (A) and cPLA₂ (B) for COX-2^+/+ and COX-2^-/- mice 24 h after icv injection of LPS or vehicle. (C) Quantification of COX-1 protein levels, relative to GAPDH internal loading control in the whole brain. (D) Representative immunoblot of COX-1 expression in COX-2^+/+ and COX-2^-/- mice 24 h after icv injection of LPS or vehicle. Data are presented as mean ± SEM (n = 4-6). ***P < 0.001 compared to the corresponding vehicle-injected mice; ^#P < 0.05 compared to the LPS-injected COX-2^+/+ mice.