Figure 3

(a) Quantification of the number of IBA-1-immunolabeled microglia with primed morphology in ventral horns ipsilateral to MCAo compared to ventral horns contralateral to MCAo in mice 24 hours and 72 hours post-reperfusion and in sham-operated mice. (b) Representative image from a ventral horn ipsilateral and (c) contralateral to MCAo 24 hours post-reperfusion immunolabelled with antibodies directed against IBA-1 for visualization of microglia. (d) Quantification of the IBA-1 immunoreactivity expressed as percent ipsilateral IBA-1-immunoreacted area 24 hours and 72 hours post-reperfusion and in sham-operated mice. (e) Representative image from a ventral horn ipsilateral and (f) contralateral to MCAo 72 hours post-reperfusion immunolabelled with antibodies directed against IBA-1 for visualization of microglia. (g) Higher magnification image of microglia in close proximity to large motor neurons in ventral horns contralateral to MCAo (magnification 100×). (h) Representative image from a ventral horn ipsilateral and (i) contralateral to MCAo immunolabelled with antibodies directed against GFAP for visualization of astrocytes (magnification 100×, same section as in g). (j) Quantification of caspase 3 activation expressed as percent ipsilateral number of caspase 3-positive motor neurons 24 hours and 72 hours post-reperfusion and in sham-operated mice. (k) Representative image from a ventral horn ipsilateral and (l) contralateral to MCAo 72 hours post-reperfusion immunolabelled with antibodies directed against active caspase 3 for visualization of apoptotic cells. All images taken at magnification 40× prior to reproduction unless noted.