Identification of Yps3p as a TLR2 ligand in 293T cells. pNiFty2-Luc transfected 293T-mTLR2 cells were treated with H. capsulatum cell wall/membrane fraction [CW], recombinant Yps3p [Yps3p], and recombinant protein H [H]. Untreated cells were used as a control for background luciferase expression [pNIFty] and heat-killed Listeria monocytogenes [HKLM] was added to cells as a positive control for TLR2 signaling. Data are presented as mean ± SD of triplicate samples and are representative of three independent experiments. Statistical analysis was performed by student's t test. *P < 0.05; **P < 0.01.