Activation of NF-κB in primary murine microglia following treatment with Yps3p. Purified microglial cells [1 × 106] from wild-type and TLR2 knockout mice were transiently transfected with pNiFty2-Luc plasmid by electroporation using the mouse macrophage nucleofector kit. The cells were incubated overnight at 37°C and 1.5 μg Yps3p was added to the cell culture medium. The microglia were harvested after 5 h and the amount of luciferase produced as a result of NF-κB activation was quantified using the bright glow substrate. Data are presented as mean ± SD of triplicate samples and are representative of three independent experiments. Statistical analysis was performed by student's t test. **P < 0.01.