Figure 3

Delayed treatment with minocycline or AG3340 dampens reactive astrogliosis. P7 rat pups were exposed to H-I and treated with either vehicle, minocycline or AG3340 once daily for 6 days, beginning 24 hours after insult. (A-C) Astrogliosis, as measured by GFAP immunoreactivity, was markedly elevated in cortical layers 1–4 (B) and layers 5–6 (C) compared to contralateral control (A) after treatment with vehicle alone. Administration of either minocycline (D-F) or AG3340 (G-I) 24 hours after H-I reduced GFAP immunoreactivity in layers 1–4 (E,H) and layers 5–6 (F,I) compared to control hemisphere (D,G). GFAP immunoreactivity was significantly reduced in cortical layers 1–4 (J) and layers 5–6 (K) of animals treated with either minocycline or AG3340 compared to treatment with vehicle alone. N = 8, * = p < 0.05. Scale bars = 100 μm.