Figure 4

Delayed treatment with minocycline or AG3340 reduces neural injury. P7 rat pups were exposed to H-I and treated with either vehicle, minocycline or AG3340 once daily for 6 days, beginning 24 hours after insult. Animals treated with vehicle alone showed robust Fluoro-Jade labeling in ipsilateral cortex and corpus striatum (B). Fluoro-Jade staining was nearly abolished after treatment with minocycline (D) and greatly reduced after treatment with AG3340 (F) relative to vehicle alone (B). No labeling was detected in contralateral control regions (A,C,E). Quantification showed a significant reduction after treatment with either minocycline or AG3340 (G) compared to treatment with vehicle alone. N = 5, * = p < 0.05. Scale bars = 100 μm.