Figure 6

Serum corticosterone levels are reduced in MCP-1^-/- mice after LPS treatment. MCP-1^-/- and MCP-1^+/+ mice were injected i.p. with LPS or saline, and serum was collected 6 hours later. Corticosterone (A) or ACTH (B) levels were measured by EIA as described in Methods. Values are represented as mean ± SEM of singlet determinations and are pooled from two independent experiments, n = 4–11 mice per group. * represents significant difference between LPS-treated MCP-1^+/+ mice and LPS-treated MCP-1^-/- mice, p < 0.01; # represents significant difference between saline-treated MCP-1^+/+ mice and LPS-treated MCP-1^+/+ mice, p < 0.001; and ≠ represents significant difference between saline-treated MCP-1^-/- mice and LPS-treated MCP-1^-/- mice, p < 0.001.