Reduction of cerebral glucose uptake in septic encephalopathy. Inflammatory gene transcription was studied using cDNA generated from whole brain lysates 24 h after intraperitoneal administration of either LPS or vehicle. Induction of the indicated pro- and anti-inflammatory cytokines was measured by real-time RT-PCR with RNA samples prepared from vehicle treated controls and LPS treated rats. Triplicate determination was performed for each mRNA sample. Gene-expression levels were normalized with GAPDH for each mRNA preparation, and the n-fold increase in LPS treated animals was calculated by comparison with the result obtained in untreated rats. Results are the mean ± (+SEM) of five animals per group. (Student's t test; *p ≤ 0.05, ***p ≤ 0.001).