Apigenin and luteolin oppose the effects of IFN-γ/CD40 ligation on TNF-α and IL-6 production in microglial cells. N9 and murine-derived primary microglial cells were seeded in 24-well tissue-culture plates (1 × 105/well) and co-treated with IFN-γ (100 U/mL)/agonistic anti-CD40 antibody (CD40 Ab; 2 μg/mL) in the presence or absence of apigenin and luteolin (25 μM) or treated with vehicle (1% DMSO; control) for 12 hrs. Cell cultured supernatants were collected and subjected to cytokine ELISA as indicated. Data were represented as mean pg of each cytokine in mg of total cellular protein (+/- SD). Results are representative of three independent experiments. (**p < 0.01).