Vaccination with mannan-Aβ
attenuates amyloid deposition in the cortical and hippocampal regions of brains. A. Representative images of hippocampal region from non-immunized controls and mice immunized with mannan-Aβ28 showing reduction of Aβ42 and Aβ40 amyloid burden or cored amyloid plaques. Aβ deposits were stained with anti-Aβ42, anti-Aβ40 antibodies, and with ThS. Original magnifications, 5× for anti-Aβ42/40 staining and 4× for ThS (scale bars = 200 μm). B, C, D. Image analysis of Aβ42 (B), Aβ40 (C) staining, and number of ThS-positive cored plaques (D) in cortex of 18–18.5 mo old Tg2576 non-immunized mice and mice immunized with mannan-Aβ28. Vaccinated mice showed significant reduction in Aβ42 (P = 0.0075), Aβ40 (P = 0.0103) burden, and in number of ThS-positive cored plaques (P = 0.0019) compared to that in non-immunized control group. Bars represent mean ± SE from n = 5 in the group of non-immunized mice, and n = 8 in the group of mice immunized with mannan-Aβ28.