Figure 2

Vaccination with mannan-Aβ ₂₈ attenuates amyloid deposition in the cortical and hippocampal regions of brains. A. Representative images of hippocampal region from non-immunized controls and mice immunized with mannan-Aβ₂₈ showing reduction of Aβ₄₂ and Aβ₄₀ amyloid burden or cored amyloid plaques. Aβ deposits were stained with anti-Aβ₄₂, anti-Aβ₄₀ antibodies, and with ThS. Original magnifications, 5× for anti-Aβ_42/40 staining and 4× for ThS (scale bars = 200 μm). B, C, D. Image analysis of Aβ₄₂ (B), Aβ₄₀ (C) staining, and number of ThS-positive cored plaques (D) in cortex of 18–18.5 mo old Tg2576 non-immunized mice and mice immunized with mannan-Aβ₂₈. Vaccinated mice showed significant reduction in Aβ₄₂ (P = 0.0075), Aβ₄₀ (P = 0.0103) burden, and in number of ThS-positive cored plaques (P = 0.0019) compared to that in non-immunized control group. Bars represent mean ± SE from n = 5 in the group of non-immunized mice, and n = 8 in the group of mice immunized with mannan-Aβ₂₈.