Figure 5
Semi-quantitative analysis of CAA in the neocortex areas of animals immunized with mannan-Aβ 28 , mannan-BSA, or non-immunized mice. A, B, C. Examples of CAA severity grades: grade 1, Aβ immunoreactivity confined strictly to the vessel wall (A); grade 2, Aβ immunoreactivity in and around vessel wall with focal infiltration of the amyloid in the neuropil (B); grade 3, extensive infiltration of amyloid into the neuropil with a complete amyloid coat around the vessel (C). Original magnification 20×, scale bar = 50 μm. D. The CAA score detected in the neocortex of mice vaccinated with mannan-Aβ28, was similar to that detected in neocortex of animals injected with mannan-BSA or non-immunized mice (P > 0.05). Bars represent mean ± SE from n = 5 in the group of non-immunized mice, n = 6 in the group of mannan-BSA immunized group, and n = 8 in the group of mice immunized with mannan-Aβ28.