Figure 5

CD45 immunohistochemistry shows hematopoietic cell activation in the subventricular zone. A) CD45 expression is barely detectable at low magnifications in control mice. B) Example of CD45 immunohistochemistry in a preclinical mouse. CD45+ cell activation was sporadic throughout the forebrain, but was consistent in the SVZ and septum. svz = subventricular zone, spt = septum. C) Sham mouse showing ramified microglia typical in all control animals. D) Preclinical mouse showing round, activated CD45^high microglia. E, F) are early onset and chronic experimental sections. Early onset section shows a mixed population of ramified, intermediate morphology and round microglia. Ramified and intermediate morphologies of microglia predominate in the chronic SVZ. Scale bar C-F = 50 μm. SVZ in C-F outlined based on high density of cells in the SVZ detected with DAPI nuclear staining. G) Higher magnification of ramified microglia in a sham animal. H) Higher magnification from an experimental animal. Scale bar G, H = 20 mm. I, J) CD45 cell activation in the hippocampus is restricted to the CA regions and excluded from the dentate gyrus.