Co-localization study revealing apoptotic bodies within degenerating neuron in ischemic TNFα-transgenic rat brain. A single 40× photomicrograph shows localization of activated caspase-3 (red) in NeuN-positive cortical neurons (green) after focal cerebral ischemia in the TNFα-transgenic (TNFα-Tg) rat. The right cerebral cortex was sampled after one hour of suture occlusion of the ipsilateral middle cerebral artery, followed by 24 hours of reperfusion. In the representative NeuN-positive neuron identified by the arrow, there is caspase-3 activation with apoptotic bodies identified by Hoechst staining (blue). Scale bar = 50 μm.