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Figure 7 | Journal of Neuroinflammation

Figure 7

From: Focal cerebral ischemia in the TNFalpha-transgenic rat

Figure 7

Regional expression of TNFα mRNA and protein in brain after focal ischemia. Two bar graphs show mean ± SD levels of TNFα protein (Panel a) and the corresponding mean ratios of mouse to rat TNFα mRNA (Panel b) in transgenic and non-transgenic rat brain with or without ischemic brain injury. TNFα-transgenic (TNFα-Tg) and non-Tg rats underwent one hour of focal brain ischemia achieved by unilateral middle cerebral artery occlusion (MCAO). Tissue samples were obtained from the developing infarct core and the border zone approximating the ischemic penumbra in both groups of animals after 3 or 24 hours of post-ischemic reperfusion. As shown in Panel a, mean TNFα levels were significantly elevated within the border zone and infarct core after 24 hours of reperfusion in both TNFα-Tg and non-Tg rats, in comparison to animals of the respective type whether non-ischemic controls or ischemic rats sampled 3 hours after reversal of MCAO (n = 3 – 4 per group; p ≤ 0.001 for all pairwise comparisons; ANOVA/Fisher's test). The mean TNFα level in the border zone adjacent to the infarct core in the transgenic rat at 24 hours was significantly higher than the comparable level in the non-Tg animal (p ≤ 0.01; ANOVA/Fisher's test). Panel b of Figure 5 shows the mean ratios of mouse and rat TNFα mRNA corresponding to the TNFα protein levels from the same animals reported in Panel a. The mean ratio of mouse/rat TNFα mRNA was significantly higher in TNFα-Tg rats compared to the non-Tg animals only under control conditions without ischemia (p ≤ 0.01; ANOVA/Fisher's test). **p ≤ 0.01; ***p ≤ 0.001

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