Figure 3
From: Astrocytes play a key role in activation of microglia by persistent Borna disease virus infection
The effects of BDV infection on expression of cytokines and chemokines in astrocyte or mixed cultures. Supernatants were analysed for cytokine/chemokine protein contents using the rat 23 cytokines/chemokines LINCOplex assay. Levels of cytokines and chemokines in media samples from BDV-infected cultures normalized to the values in obtained from respective mock-infected sister cultures. Media samples were collected from n = 6 mixed astrocyte/neuron/microglia cultures at 12–14 d.p.i. and from n = 4 persistently infected astrocyte cultures at 7–10 days after passage.