Expression of TLR12 in brains of mock infected control and NCC mice analyzed by immunofluorescence microscopy. Balb/c mice were infected intracranially with M. corti and sacrificed at various times p.i. Brain cryosections were analyzed for expression of TLR12 and cell specific markers using fluorochrome conjugated antibodies. Nuclear staining DAPI is blue. (A) Mock infected control stained with TLR12 specific antibody (400×). (B) M. corti infection at 1 wk showing TLR12 positive staining (400×). (C) M. corti infection at 3 wk when maximum TLR12 expression was observed (400×). (D) M. corti infection at 6 wk showing TLR12 positive staining (400×). (E) Mock control mice stained with TLR12 and neuron specific antibody (Neun) (400×). The insert e' represents a 2× magnification of a selected area depicting TLR12 expression (Red) on neurons. (F) M. corti infection at 3 wk showing TLR12 positive neurons, merged image (yellow/orange; 400×). (G) The relative levels of TLR12 expression in mock and M. corti infected animals was calculated as described in experimental procedures.