Figure 3

Aβ1–42 stimulated a conformation-specific increase in proinflammatory cytokine and chemokine secretion. Primary microglia were unstimulated (control) or stimulated for 24 hours with 20 μM Aβo, 20 μM Aβf, or 25 ng/mL lipopolysaccharide (LPS, positive control). (A) Media were collected and used to perform a preliminary screen of a mouse inflammation antibody array to assess whether relative differences in secretion occurred. In order to quantitate select changes, media was collected and analyzed by (A) mouse IL-6 ELISA, (B) mouse KC ELISA, or (C) mouse MCP-1 ELISA. Data were analyzed by unpaired ANOVA with Tukey's post-test comparison and are expressed as mean +/- SD. Values are representative of three independent experiments (* = p < 0.05 over control, *** = p < 0.001 from control, ** = p < 0.001 from oligomer, # = p < 0.001 from fibril).