Figure 4
From: Beta amyloid oligomers and fibrils stimulate differential activation of primary microglia
Aβ1–42 oligomers were only neurotoxic in neuron-microglia co-cultures. (A) Primary microglia, (B) 14 day in vitro primary cortical neuron, or (C, D) primary neuron-microglia co-cultures were unstimulated (control) or stimulated for 24 (A-C) or 48 (D) hours with increasing doses of Aβo, Aβf, or vehicle. Media were collected and analyzed by (A-C) LDH release assay to assess viability. Data were analyzed by unpaired ANOVA with Tukey's post-test comparison and are expressed as mean +/- SD. Values are representative of three independent experiments (* = p < 0.05 from control, ** = p < 0.001 from control, *** = p < 0.01 from Aβf+neurons). (D) Alternatively, neurons were fixed after stimulation and immunostained for MAP2 expression and counted to assess viability. Data were analyzed by unpaired ANOVA with Tukey's post-test comparison, expressed as mean +/- SEM, and representative of three independent experiments. (* = p < 0.01 from microglia+neurons, ** = p < 0.001 from microglia+neurons).