Cortical neuron cultures were pre-incubated (32 h) with acetaminophen (0–300 μM) (APAP) and oxidatively stressed with menadione (5 μM for 4 h). Total protein was extracted and western blot analysis performed using specific antibodies for cleaved caspase3 (Fig. 5a) and Bcl2 (Fig. 5b). Blots probed with GAPDH were used to confirm loading equivalency. Relative expression determined from densitometric scans from 3 separate experiments are shown below each blot. #p < 0.001 vs. control; *p < 0.05 vs. 0 μM APAP; **p < 0.01 vs. 0 μM APAP; ***p < 0.001 vs. 0 μM APAP.