Primary cortical neurons were incubated with 100 μM of APAP (32 h) and neutralizing Bcl2 antibody for 2 h and then exposed to oxidative stress (5 μM menadione for 4 h). Cell survival assayed with MTT reagent. In each experiment, the number of control cells i.e. viable cells not exposed to any treatment, was defined as 100%. Inset shows the neutralization of protein expression of Bcl2 with neutralizing anti-human Bcl2 antibody. *** p < 0.001 vs. control. # p < 0.01 vs. menadione. δ p < 0.001 vs. m+APAP (menadione + APAP).