Figure 2
[Nα-Bodipy]-des-Arg9-BK (BdABK) selectivity for B 1 R was evaluated by confocal microscopy. While B1R labeling in the presence of BdABK was absent in thoracic spinal cord of control rats (A), it was shown as green dots in STZ-treated spinal cord (B, and enlarged in C). B1R labeling was absent in STZ-treated spinal cord when BdABK (50 μM) was co-incubated with 10-5M SSR240612 (D) or 10-5M R-715 (E). Background staining represents non specific autofluorescence. Scale bars = 20 μm (A, B, D, E) and 8.2 μm (C). Pictures are representative of a minimum of 4 sections per rat from 4 different STZ-diabetic rats.