Figure 2
From: Modulation of inducible nitric oxide synthase expression by sumoylation
Changes in SRG, NOS2, and C/EBPβ mRNA levels during aging. Cerebral cortices of C57BL/6 mice of the indicated ages were sonicated briefly in Trizol reagent, total mRNA extracted, and converted to cDNA. Aliquots were analyzed by QPCR for levels of (A) SUMO-1 and Ubc9; and (B) NOS2 and C/EBPβ mRNAs. The data are mean ± sd of 3 mice per age group, normalized to values for β-actin mRNA measured in the same samples. There was a significant decrease in SUMO-1 and Ubc9, and a significant increase in NOS2 and C/EBPβ over time (P < 0.01, 1-way ANOVA); *, p < 0.05; **, p < 0.01 vs 3-month samples (Bonferroni multiple comparison post hoc tests).