IL-17RA expression in the CNS of EAE mice. (A) Clinical profile of EAE. Female C57BL/6 mice (n = 8) were immunized with MOG35–55 and scored daily. Data represent mean clinical scores ± SEM. One experiment of three is shown. (B-G). CNS inflammation and demyelination. Mice were sacrificed at day 20 p.i., spinal cords were harvested and 5 μm sections were stained with H&E (B, D, F) or LFB (myelin stain; C, E, G). Magnifications are 40× (B-E) and 100× (F, G). EAE mice had significant cellular infiltration (arrows; D, F) and demyelination (arrows; E, G). No inflammation or demyelination occurred in control mice (B, C). (H) IL-17RA expression is up-regulated in the inflamed CNS of EAE mice. EAE and control mice (n = 5) were sacrificed at day 20 p.i. and IL-17RA expression from isolated spinal cords was assessed by RT-PCR using a primer set from exon boundary 1–2. Expression of IL-17RA in EAE mice is upregulated > 5-fold (*** P < 0.0001).