Figure 2

Effects of IFN-γ, LPS, and GW 501516 on PPAR-β and PPAR-γ mRNA expression. GW 501516 (black bars) up-regulated the expression of PPAR-β (A) and PPAR-γ (B) in control cultures and in cultures treated with the inflammatory agents. Cultures received GW 501516 (5 μM) 18 hours before the addition of the inflammatory agents, and again together with INF-γ (50 U/ml) and LPS (5 μg/ml). Cultures were harvested 48 hours after the inflammatory treatment. Values are expressed as fold change relative to the untreated control cultures (= 1), each value being the mean of 7 replicate cultures. Results were statistically evaluated for significance by the Kruskal-Wallis test followed by the Mann-Whitney test. (°°°P < 0.01 compared with cultures not treated with GW 501516).