Figure 5
Effects of antibody-mediated demyelination and GW 501516 on GFAP, TNF-α, and IL-6 mRNA expression. The antibody-mediated demyelination induced a significant increase of GFAP mRNA (A), but did not affect TNF-α (B) nor IL-6 (C) mRNA expression. Cultures received GW 501516 (5 μM) 18 hours before and again together with the demyelinating agents. Cultures were harvested 48 hours after the demyelinating treatment. Values are expressed as fold change relative to the untreated control cultures (= 1), each value being the mean of 6 replicate cultures. Results were statistically evaluated for significance by using the Kruskal-Wallis test followed by the Mann-Whitney test (**P < 0.01 compared with untreated control cultures; °P < 0.05, °°P < 0.01 compared with cultures not treated with GW 501516).