Figure 1

RelA nuclear translocation in neurons after glutamate treatment. Nearly pure neocortical neurons were treated with glutamate (50 μM) for the indicated times and cells were harvested for fractionation (Cyto.: cytosolic fraction; Nuc.: nuclear fraction); these were probed by western blot for RelA. Whole-cell lysates were also prepared from either astrocyte or neuron cultures; these were probed by western blot for IκBα (Whole: neuron whole cell lysate). TNFα was applied to the astrocytes at 100 ng/ml for the indicated times (min).