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Figure 2 | Journal of Neuroinflammation

Figure 2

From: Unique aspects of transcriptional regulation in neurons – nuances in NFκB and Sp1-related factors

Figure 2

Glutamate is unable to activate neuronal NFκB in mixed cell cultures. Neocortical neurons from κB/β-gal transgenic mice were either plated on the top of wild-type astrocytes ("+glia") or retained as nearly pure neuronal cultures ("-glia"). A. Glutamate was applied at 50 μM for 10 min, then chased for the times indicated (h). B. Glutamate was applied at 20 μM continuously for the times indicated (h). β-gal activity was determined by a luminescent assay. Values represent activity relative to untreated cultures ± SEM in quadruplicate cultures. ANOVA followed by Scheffe post-hoc test showed significance between control and each treatment, and between the "+glia" and "-glia" in all comparisons except the 6 h timepoint in A. (p < 0.02).

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