Figure 5From: Absence of the complement regulatory molecule CD59a leads to exacerbated neuropathology after traumatic brain injury in miceRegulation of intracerebral gene expression for apoptotic mediators. Total RNA was extracted from homogenized murine brains (injured left hemispheres) at 4 h, 24 h, and 7 days after sham surgery or experimental head injury. Gene expression levels of Fas, FasL, Bax and Bcl-2 were determined by semi-quantitative two-step real-time RT-PCR, as described in the methods section. A significant induction of Fas gene expression was detected at 4 h in both trauma groups, compared to sham-operated controls (*P < 0.05, unpaired Student's t-test). In addition, head-injured wild-type mice showed a significant induction of Bcl-2 expression at all time-points after trauma, compared to sham-operated controls (*P < 0.05). No significant differences between head-injured wild-type and CD59a-/- mice were detected for any gene at any time-point. Data are shown as mean ± SEM for n = 6 per group and time-point. TBI, traumatic brain injury; WT, wild-type.Back to article page