Effect of DEDA on IR injury-induced BBB leakage and edema. Representative photographs showing extravasation of Evan's blue (EB) in six coronal sections of brain (A). Spectrofluorimetric estimation of EB in six coronal sections (B). Significant EB leakage was observed in Veh brain and the leakage was reduced in the DEDA-treated group. EB extravasation was not observed in Sham animals. Treatment with DEDA decreased the brain water content in ipsilateral brain (C). Closed bars represent ipsilateral and open bars represent contralateral hemispheres. Data are expressed as means ± SD from triplicate determinations of three different samples in each group. *** p < 0.01 vs. Sham, ** p < 0.01 vs. Veh.