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Figure 4 | Journal of Neuroinflammation

Figure 4

From: Expression of P2 nucleotide receptors varies with age and sex in murine brain microglia

Figure 4

P2Y12 (A), P2Y13 (B), and P2Y1 (C) mRNA expression increases with age. P2R mRNA levels in freshly-isolated microglia from male and female mice of different ages and mixed-sex primary neonatal microglial cultures were determined using qRT-PCR. Expression was normalized to β-actin and average folds (+/- SEM) are graphed relative to the 3d time point of each sex. n = 3–6; for primary cells n = 5 separate culture preparations. Values in parentheses are fold-changes of the lowly-expressed samples. # 0.05 < p ≤ 0.10; * p ≤ 0.05; ** p ≤ 0.01; *** p ≤ 0.005. Primary cultures: †† p ≤ 0.01 vs. all freshly-isolated microglia; a - 0.05 < p ≤ 0.1 vs. 21 d, 7 wk, 4 mo, 12 mo and p ≤ 0.05 vs. 3 d; b - p ≤ 0.05 vs. 7 wk and 4 mo; c - p ≤ 0.01 vs. 21 d and 4 mo. N.D. = not detected.

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