Table 1 Primer sequence table
From: Expression of P2 nucleotide receptors varies with age and sex in murine brain microglia
Gene | Forward primer | Reverse primer | Average Ct (vs. β-actin) for 3d samples |
|---|---|---|---|
P2X1 | CAGAAAGGAAAGCCCAAGGTATT | CACGTCTTCACAGTGCCATTG | +9 |
P2X2 | GCTGCTCATTCTGCTTTACTTCG | TCCCACACTTTGTGTTCCGA | N.A. |
P2X3 | AAGGCTTCGGACGCTATGC | GATGACAAAGACAGAAGTGCCCT | +16 |
P2X4 | AGACGGACCAGTGATGCCTAAC | TGGAGTGGAGACCGAGTGAGA | +6 |
P2X5 | GATGTGGCAGACTTTGTCATTCC | CCTTCACGCTCAGCACAGATG | +4 |
P2X6 | ACGTGTTCTTCCTGGTAACCAACT | TGGACATCTGCCCTGGACTT | +13 |
P2X7 | ACAATGTGGAAAAGCGGACG | TCAATGCACACAGTGGCCA | +4 |
P2Y1 | AGCAGAATGGAGACACGAGTTTG | GGGATGTCTTGTGACCATGTTACA | +16 |
P2Y2 | GAAGAACTGGAGCAGGCGCT | CCATTGCCCTGGACCTGATC | +12 |
P2Y4 | CTGCAAGTTCGTCCGCTTTC | GTATTGCCCGCAGTGGATG | +16 |
P2Y6 | TGAAAACAACGAGGAACACCAA | CAGCCTTTCCTATGCTCGGA | +6 |
P2Y12 | CACAGAGGGCTTTGGGAACTTA | TGGTCCTGCTTCTGCTGAATC | +3 |
P2Y13 | CAGCTGAGTCTCTTCCAAAACAAA | TGCATCCCAGTGGTGTTGAT | +5 |
P2Y14 | CCACCACAGACCCTCCAAAC | CAACACGGGAATGATCTGCTTT | +14 |