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Table 2 In vitro culturing of microglia alters their P2 receptor expression profiles.

From: Expression of P2 nucleotide receptors varies with age and sex in murine brain microglia

Gene Fold 1° vs. 3d
  Ave. of ♂ and ♀ (+/- SEM)
P2X1 ↓167 (+/- 83) ***
P2X3 ↑ 5.39 (+/- 2.07)
P2X4 ↑ 2.95 (+/- 0.43) *
P2X5 ↓ 833 (+/- 347) ***
P2X6 ↑ 1.52 (+/- 0.32)
P2X7 ↓ 2.65 (+/- 0.38) ***
P2Y1 ↑ 12.3 (+/- 4.61) #
P2Y2 ↑ 11.1 (+/- 4.41) #
P2Y4 ↑ 4.54 (+/- 2.41)
P2Y6 ↓ 1.47 (+/- 0.14) **
P2Y12 ↓ 90.9 (+/- 16.5) ***
P2Y13 ↓ 100 (+/- 20.0) ***
P2Y14 ↑ 2.22 (+/- 0.20) ***
  1. P2R mRNA levels in freshly-isolated microglia from 3d male and female mice and mixed-sex primary neonatal microglial cultures (also derived from 3d mice) was determined using qRT-PCR. Expression was normalized to β-actin and the average fold change (+/- SEM) of the mixed primary cultures is given compared to the average of male and female 3d freshly-isolated microglia. n = 3–4 for freshly-isolated samples; n = 4–5 separate culture preparations for primary cells. # p < 0.10, * p < 0.05, ** p < 0.01, *** p < 0.001.