Expression of P2 nucleotide receptors varies with age and sex in murine brain microglia

Table 2 In vitro culturing of microglia alters their P2 receptor expression profiles.

P2R mRNA levels in freshly-isolated microglia from 3d male and female mice and mixed-sex primary neonatal microglial cultures (also derived from 3d mice) was determined using qRT-PCR. Expression was normalized to β-actin and the average fold change (+/- SEM) of the mixed primary cultures is given compared to the average of male and female 3d freshly-isolated microglia. n = 3–4 for freshly-isolated samples; n = 4–5 separate culture preparations for primary cells. # p < 0.10, * p < 0.05, ** p < 0.01, *** p < 0.001.

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