A-F: Representative photomicrographs of c-Fos immunoreactivity in coronal sections of the PVN, ARC and SON. Images derived from saline-injected (A-C) and LPS-treated (D-F) rats. G-L: Analysis of c-Fos and nesfatin-1 (green) double-labeling in coronal sections through the hypothalamus of LPS-treated rats. Dashed boxes in low magnification images (G-I) indicate the area where high magnification images (J-L) originate. Arrowheads: c-Fos+/Nesf+ neurons; arrows: c-Fos+ neurons negative for nesfatin-1. ARC arcuate nucleus; ox, optic chiasm; PVN, paraventricular nucleus of the hypothalamus; SON: supraoptic nucleus; 3V, third ventricle. Scale bars: 200 μm (A-I), 50 μm (J-L).