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Figure 5 | Journal of Neuroinflammation

Figure 5

From: Cerebellar Purkinje cells incorporate immunoglobulins and immunotoxins in vitro: implications for human neurological disease and immunotherapeutics

Figure 5

Cultures incubated with GK1.5 IgG-daunorubicin immunotoxin or with GK1.5 antibody control, scored for cell death. Cultures were incubated with either the immunotoxin (Panel A) or control IgG (Panel B) for 8 hours. SYTOX green was added during the last two hours of incubation to detect cell death prior to fixation and immunostaining for IgG. Cy5 labeling of IgG appears red. Intracellular presence of SYTOX, indicating cell death, appears green. Colabeling for IgG and SYTOX appears yellow. The SYTOX green channel is shown for the immunotoxin (Panel C) and control IgG (Panel D). Examples of cells scored as live cells (positive for IgG and negative for SYTOX green) are indicated with gray horizontal arrows, while examples of dead cells positive for IgG and SYTOX green are indicated with a white vertical arrow. (Objective magnification 40×)

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Journal of Neuroinflammation

ISSN: 1742-2094