Administration of S-nitrosoglutathione after traumatic brain injury protects the neurovascular unit and reduces secondary injury in a rat model of controlled cortical impact

Table 1 Effect of CCI and protection by GSNO on BBB permeability assessed by macromolecular tracer dye Evan's blue.

Group	# of animal showing staining/total # of animals
	0	+	++	+++
Sham	5/5	0/5	0/5	0/5
CCI+VEH	0/7	1/7	3/7	3/7
CCI+GSNO	0/7	4/7	2/7	1/7

Qualitative assessment of Evan's blue (EB) dye using a standard procedure of grading as described by Rapoport et al [77] and as given: 0 = no staining; + = mild staining; ++ = moderate staining; +++ = heavy staining. Experimental details of EB extravasation are described in Methods. Animals were treated with GSNO (0.05 mg/kg) at 2 h following CCI. CCI+VEH and Sham animals were treated with the vehicle (PBS). n = 5 (Sham) and n = 7 (CCI+VEH and CCI+GSNO).

ISSN: 1742-2094