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Table 1 Changes in early gene expression: neurotransmitters, ion channels and exchangers, apoptosis, mitochondria and glutathione metabolism

From: Differential effects of Th1, monocyte/macrophage and Th2 cytokine mixtures on early gene expression for molecules associated with metabolism, signaling and regulation in central nervous system mixed glial cell cultures

NEUROTRANSMITTERS AND RECEPTORS Th1 M/M Th2
S50879 acetylcholinesterase T subunit   2.07*  
AF050662 activity and neurotransmitter-induced early gene 10   -3.89* -5.27**
AF050664 activity and neurotransmitter-induced early gene 12   2.79*  
AF050661 activity and neurotransmitter-induced early gene 9 -2.91* -4.64** -3.71*
X57659 adrenergic receptor, alpha 2 c-4 2.53***   
M16406 cholinergic receptor, muscarinic m1   -2.05* -2.07*
J05231 cholinergic receptor, nicotinic, alpha 5   -2.29***  
L08227 cholinergic receptor, nicotinic, alpha 6 -3.26*   
L31619 cholinergic receptor, nicotinic, alpha 7   -2.14** -2.29**
L31622 cholinergic receptor, nicotinic, beta 2   -2.33**  
M35077 dopaminergic receptor A1 -12.11***   -14.19***
A17753 dopaminergic receptor D3 -10.31*** -5.21* -8.28**
L08493 GABA-A receptor, alpha 4   -2.0*  
S56679 glutamate receptor, AMPA-selective A    -3.31***
U08255 glutamate receptor, ionotropic, delta 1 2.68****   
AF027331 glutamate receptor, ionotropic, kainate 5   2.15*  
D13211 glutamate receptor, ionotropic, NMDA 2A   -3.02** -3.50***
X96790 glutamate receptor, metabotropic 7b. -9.46**** -3.02** -3.50***
D16817 glutamate receptor. metabotropic 7   -2.19*  
U28504 glutamate transporter, vesicular, family 17 -3.29*   
X55246 glycine receptor, alpha 1    -3.05****
D00833 glycine receptor, alpha 1 subunit -2.21* -7.08****  
X57281 glycine receptor, alpha 2 subunit -2.36***   
L13600 glycine transporter 1 2.86***   2.12***
U66274 neuropeptide Y receptor 5 -18.76** -8.72*  
X56306 preprotachykinin A (substance P precursor)    -7.17**
L46874 proton-driven peptide transporter. -2.10** -3.50***  
X90561 purinergic receplor P2X, ligand-gated 3    -5.56*
X80477 purinergic receptor P2X, ligand-gated 1 -3.23*   -3.0***
AF020758 purinergic receptor P2X, ligand-gated 2   -3.86* -3.81*
X95882 purinergic receptor P2X, ligand-gated 7   -2.10*  
U56839 purinergic receptor P2Y, G-protein coupled 2   3.51*** 2.41***
Y311433 purinergic receptor P2Y, G-protein coupled 4    -2.53*
X66842 serotonergic receptor 2B -2.09***   
U20907 serotonergic receptor 4    -6.05****
ION CHANNELS
M99222 Ca++ channel, A/P type, alpha 1, splice variant -3.75*   
M57682 Ca++ channel, voltage-gated, L type, alpha 1D -4.62*** -3.39* -7.36***
D26111 Cl- channel (ClC-K2L and ClC-K2S), splice variant -3.13* -2.02*  
X62894 Cl- channel, voltage-gated, 1 (skeletal muscle)   -2.80** -2.59*
X78461 K+ inwardly-rectifying channel, J12 (kir 3.3/IRK3)   2.05*  
U69884 K+ channel, small conductance Ca++-activated -3.17*   -2.55*
L35771 K+ channel, inwardly-rectifying, J5    -3.56***
L77929 K+ channel, inwardly-rectifying, J9    -2.49*
U40603 K+ channel, large conductance Ca++activated M alpha1 -3.50*   -3.61*
AB010963 K+ channel, large conductance Ca++-activated M beta1    -2.20***
D10709 K+ channel, lsk1 (epithelial), E1    2.50***
AF031384 K+ channel, voltage-gated, K3   -2.45* -2.36**
AF087453 K+ channel, voltage-gated, KQT-like 2   -3.39*  
AF087454 K+ channel, voltage-gated, Q3   -2.56* -2.18*
Y17606 K+ channel, voltage-gated, S1 -4.17** -3.80* -4.68*
J04731 K+ channel, voltage-gated, shaker related 2 -7.17*   
X16003 K+ channel, voltage-gated, shaker related 2.   -2.44*  
U72410 K+ inwardly-rectifying channel, J3 (GIRK1)    2.75***
M22253 Na+ channel, voltage-gated, 1 alpha -9.23*** -6.92**  
M91808 Na+ channel, voltage-gated, 1 beta    -2.46****
AF000368 Na+ channel, voltage-gated, 9 alpha -3.36*   
AA891751 Na+ channel, voltage-gated, 3 alpha (CIN3)    -2.10**
Y00766 Na+ channel, voltage-gated. 3 alpha (CIN3)    -2.76**
ATPase ION EXCHANGERS
M99223 Ca++ ATPase, cardiac, fast twitch 1 -3.80** -2.82* -2.70**
AA800212 Ca++ ATPase, cardiac, slow twitch 2 -2.06*   -2.35*
Al172499 Ca++ ATPase, plasma membrane 1   3.0***  
X76452 Ca++ ATPase, plasma membrane 4    -2.06***
AA956437 ER ATPase, peroxisome biogenesis factor 1 -2.35*   
U94911 H+/K+ ATPase, nongastric, alpha 2a   -2.30***  
M90398 H+/K+ ATPase, nongastric, alpha 2a   -2.73*  
U15176 Na+/K+ ATPase, alpha 4 -3.15*   -2.86*
APOPTOSIS
H31839 Bcl X 2.35**   
AF025671 caspase 2   -2.93**** -2.63***
AF072124 caspase 7 3.71*** 3.17**  
M33605 cytolysin -2.06***   -2.22***
Al639313 huntingtin associated protein interactive protein -2.40*** -2.34*  
Al176462 programmed cell death 2   -2.47****  
MITOCHONDRIA
AJ007488 16s ribosomal RNA, mitochondrial -4.1****   -2.81**
X72758 COX VIa2, Complex IV -3.16*   
M20183 COX VIc1, Complex IV -3.76**   
M10140 creatine kinase (muscle)   -2.02* -2.01*
Al044488 ferredoxin 1 -2.27*   -2.61*
D26393 hexokinase II 2.75*   
X87884 mitochondrial capsule selenoprotein.    -2.88*
AA799479 NADH dehydrogenase (ubiquinone), Complex I   -2.43***  
AA891651 NADH dehydrogenase (ubiquinone), Complex I -2.20* -2.61***  
AI75973 NADH dehydrogenase (ubiquinone), Complex I    -2.61*
X59736 sarcomeric mitochondrial creatine kinase.   -5.18*  
Y00497 superoxide dismutase 2 3.79*** 5.82**** 2.00**
X68041 superoxide dismutase 3 -2.96**   
X59793 ubiquitous mitochondrial creatine kinase   -2.61**  
A04674 uncoupling protein 1, proton carrier -2.19***   
GLUTATHIONE-RELATED
U73174 glutathione reductase 2.07* 2.74**  
AI138143 glutathione S-transferase, theta 2 -2.04**   
S72506 glutathione S-transferase, Yc2 subunit   -5.0** -3.01*
AI235747 glutathione-S-transferase, alpha (Ya)   -2.36* -2.22***
M81855 P-glycoprotein, multi-drug resistance 1 5.45** 5.10***  
  1. Values represent averages of fold changes from three separate experiments for each cytokine mixture compared to control. The accession numbers are from Genbank.
  2. ****p < 0.01; ***p < 0.05; **p < 0.10; *p < 0.20.
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