Differential effects of Th1, monocyte/macrophage and Th2 cytokine mixtures on early gene expression for molecules associated with metabolism, signaling and regulation in central nervous system mixed glial cell cultures

Table 3 QRT-PCR validation of gene array results for cytokine-induced changes in gene expression at 6 hours

Gene	Cytokine	Array	PCR
iNOS
	Th1	14.7	35.9 +/- 18.8
	M/M	14.6	371 +/- 146
	Th2	NC	-3.7 +/- 0.9
arginase
	Th1	NC	1.05 +/- 0.28
	M/M	NC	5.37 +/- 2.03
	Th2	4.82	20.9 +/- 5.16
P glycoprotein
	Th1	5.4	2.51 +/- 2.54
	M/M	5.1	3.77 +/- 1.84
	Th2	NC	1.99 +/- 0.28

RNA extracts from 2 experiments for iNOS and 3 experiments for arginase and P glycoprotein were analyzed by QRT-PCR, as described in Methods. Values are averages +/- range. In agreement with the gene array results, Th1 and M/M cytokines markedly upregulated expression of iNOS in the mixed glial cultures; Th2 cytokines upregulated arginase; and Th1 and M/M cytokines upregulated P glycoprotein. Differences from the gene array were a decrease in iNOS by Th2, an increase in arginase by M/M and an increase in P glycoprotein by Th2 cytokines.
Values for iNOS, +/- range; values for arginase and P glycoprotein, +/- S.D.

ISSN: 1742-2094