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Table 3 QRT-PCR validation of gene array results for cytokine-induced changes in gene expression at 6 hours

From: Differential effects of Th1, monocyte/macrophage and Th2 cytokine mixtures on early gene expression for molecules associated with metabolism, signaling and regulation in central nervous system mixed glial cell cultures

Gene Cytokine Array PCR
iNOS    
  Th1 14.7 35.9 +/- 18.8
  M/M 14.6 371 +/- 146
  Th2 NC -3.7 +/- 0.9
arginase    
  Th1 NC 1.05 +/- 0.28
  M/M NC 5.37 +/- 2.03
  Th2 4.82 20.9 +/- 5.16
P glycoprotein    
  Th1 5.4 2.51 +/- 2.54
  M/M 5.1 3.77 +/- 1.84
  Th2 NC 1.99 +/- 0.28
  1. RNA extracts from 2 experiments for iNOS and 3 experiments for arginase and P glycoprotein were analyzed by QRT-PCR, as described in Methods. Values are averages +/- range. In agreement with the gene array results, Th1 and M/M cytokines markedly upregulated expression of iNOS in the mixed glial cultures; Th2 cytokines upregulated arginase; and Th1 and M/M cytokines upregulated P glycoprotein. Differences from the gene array were a decrease in iNOS by Th2, an increase in arginase by M/M and an increase in P glycoprotein by Th2 cytokines.
  2. Values for iNOS, +/- range; values for arginase and P glycoprotein, +/- S.D.
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