Figure 1
CCR2 and CCL2 expression in the hippocampus. Confocal micrographs showing CCR2 staining in control (A) and SE (B) rats. Note that both control and SE rats presented a similar punctate staining pattern of cell bodies (arrows). In addition to cell body staining, SE rats presented numerous isolated processes labeled with CCR2 (arrowheads). (C) Graph of the mean density of CCR2-labeled cell bodies showing that the density of CCR2 labeled cells in SE rats was significantly increased (mean Ā± SEM, **p < 0.01, t-test) in hippocampal CA1 when compared with control rats. (D) Graph of the mean CCL2 protein expression in the hippocampus showing significantly increased (mean Ā± SEM, **p < 0.01, t-test) CCL2 expression compared to control rats. Note that there was no change in CCL2 expression in the cerebellum, a region where monocyte infiltration is not expected after SE. Scale bar = 20 Ī¼m.