Figure 3

Stereological analysis of CCR2-labeled neurons in the hippocampus. (A) The density of CCR2/NeuN double-labeled cells in different regions of the hippocampus. Note that SE rats had a significantly (mean ± SEM, *p < 0.05, t-test) increased density of CCR2/NeuN double-labeled cells in CA1when compared with control rats. Although the graphs appear to show a trend towards an increased density of CCR2/NeuN in the other hippocampal regions, none of them approached significance. (B) Graph depicting the mean proportion of CCR2+ cells that were co-labeled with NeuN. Following SE, the proportion of CCR2-labeled cells that double-label for NeuN was significantly (mean ± SEM, *p < 0.05, t-test) decreased in the molecular layer and granular cell layer (ML/GCL) of the dentate gyrus and CA1 region of the hippocampus. This suggests that other cell types are induced to express CCR2 following seizures.