Figure 6

Confocal micrographs of double-labeling for CCR2 and Iba1 in the hippocampus. In control rats (A-C), microglia cells (arrowheads) in the hippocampus do not present a clear relationship with CCR2+ cells (arrow). On the other hand, rats that experienced SE (E-F) shows many microglial cells (arrowheads) that appear activated and are closely apposed to CCR2-labeled cells (arrow). (G) Graph of the distance between CCR2+ and Iba1+ cells. Note that compared to controls, SE rats present a significant decrease in the distance between these two cells types, with the mean distance being less than a few micrometers (mean ± SEM, **p ≤ 0.001, Mann-Whitney Rank Sum Test). Scale bar: 20 μm.