Figure 5

Inhibition of migration with PTx, heating, or filtration of substances >3 kDa. CSF samples of LNB patients (n = 3, black bars) or 500 ng/ml rhCXCL13 (mean of two independent experiments, done in duplicates, gray bars) were used as chemotactic agent. Either, the B-cells were preincubated with PTx, or the chemotactic agent was either filtrated to eliminate substances larger than 3 kDa or heated at 60°C prior to the migration assay. The reduction of migration was compared to the untreated B cells, or the untreated chemotactic agents, respectively.